SubtiBank SubtiBank
clpX [2017-07-30 17:38:52]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

clpX [2017-07-30 17:38:52]

AAA unfoldase, ATP-dependent Clp protease, ATP-binding subunit (class III heat-shock protein)
locus
BSU28220
pI
4.64
mw
46.25 kDa
protein length
420 aa Sequence Blast
gene length
1260 bp Sequence Blast
function
protein degradation
product
AAA unfoldase, ATP-dependent Clp protease, ATP-binding subunit
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.7|Proteolysis] → [category|SW 3.3.7.4|Additional proteins involved in proteolysis]

    • Gene

    Coordinates
    2,884,781 → 2,886,043

    Phenotypes of a mutant

  • increased thermotolerance due to increased stabiliy of [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx] and thus increased expression of ''[gene|4E5C84FDC8FE2FEFF47306C91ECAB7F17D3E38E9|trxA]'' [Pubmed|24417481]
  • the mutation suppresses the heat sensitivity of spores overexpressing ''[gene|85DE3CB6CDA61C141C6030367C0A13BB642160B9|cmpA]'' [Pubmed|26387458]

    • The protein

    Catalyzed reaction/ biological activity

  • ATPase/chaperone

    • Protein family

  • clpX chaperone family (according to Swiss-Prot) ClpX (IP004487) [http://www.ebi.ac.uk/interpro/IEntry?ac=IPR004487 InterPro], AAA -type ATPase (IPR013093) [http://www.ebi.ac.uk/interpro/IEntry?ac=IPR013093 InterPro] (PF07724) [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]

    • Paralogous protein(s)

  • [protein|86A2F2F65290F4471D6FD03B694821C66C180D8A|ClpC], [protein|8C5B14FE5E03427F9A598C75D4081FA0D6696299|ClpE]

    • [SW|Domains]

  • AAA-ATPase [http://pfam.sanger.ac.uk/family?acc=PF07724 PFAM]
  • Zinc finger [http://pfam.sanger.ac.uk/family?acc=PF06689 PFAM]

    • Structure

  • [PDB|1UM8] (from ''Helicobacter pylori'') [Pubmed|14514695]

    • [SW|Localization]

  • cytoplasmic polar clusters, excluded from the nucleoid, induced clustering upon heat shock, colocalization with [protein|CB06A70DE7462CEB7AF5D8C28943C878DD56DE1A|ClpP] [Pubmed|18786145,18689473]
  • [[File:ClpX.jpg‎ ]]

    • Expression and Regulation

    Operons

    genes
    [gene|297F53DAD3351E0C55108DD2C93B78FFB174438C|clpX]
    description
    [Pubmed|11325926]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|8973311], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|908DB17A39D518E84977250C55825E77FA02E391|CtsR]: repression, [Pubmed|9852015], in [regulon|908DB17A39D518E84977250C55825E77FA02E391|CtsR regulon]

    • regulation

  • induced by heat stress ([protein|search|CtsR]) [Pubmed|9852015]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B019 (clpX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1018 NBRP B. subtilis, Japan]
  • GPUG2 (aphA3), available in [SW|Ulf Gerth]'s and [SW|Jörg Stülke]'s labs
  • GP1787 (aphA3), available in [SW|Jörg Stülke]'s lab
  • ''clpX::kan'', ''clpX::spec'' and ''clpX::cat'' available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen]] Lab

    • GFP fusion

  • C-terminal GFP fusions (both single copy and 2th copy in ''amyE'' locus, also as CFP and YFP variants) available from the [http://subtiwiki.uni-goettingen.de/wiki/index.php/Leendert_Hamoen Hamoen]] Lab

    • Labs working on this gene/protein

  • [SW|Leendert Hamoen], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/l.hamoen homepage]

    • References

    Reviews

  • 23375660,19680248,17302811,23479438,19609260,26639779

    • Original Publications

  • 12761164,10809708,9643546,11807061,14679237,18689476,16899079,8973311,19136590,11325926,8973311,9852015,18689473,20525796,15948963,18786145,24417481,24942655,25433860,25866879,14514695,26387458,27669037